Pre-adipocyte cell fate reprogramming

Adipose tissue secretes cytokines to regulate essential functions, but comprehensive study is prevented by difficult-to-access depots such as visceral epicardial adipose tissue and differences between donor sources and methods to generate cell lines. This project will start with an isogenic population of cultured human preadipocytes and reprogram them to specific types of adipocytes using a combinatorial process analogous to that of induced pluripotent stem cells. This project includes working with single cell (sc)-RNAseq and sc-ATACseq datasets using XGBoost and other models.