Protein tagging at scale to generate a comprehensive map of the cell

We have recently reported a method for generating pools of cells, where each cell in the pool has a different protein tagged with either an affinity reagents (e.g. FLAG) or fluorescent protein (e.g. mCherry). Using this method, we are collaborating with several other groups on campus to generate a comprehensive map of the cell, elucidating the complexity in protein-nucleic acid and protein-protein interactions at scale and across genotype and environmental conditions.