RNA regulation of immunity

RNA epigenetics or epitranscriptomics is an emerging field focused on chemical modifications in RNA. We are interested in understanding how RNA modifications affect the immune system during viral infections, vaccine development, immunotherapy, and in cancer. We employ in vivo models as well as non-human primates and human tissues to investigate genetics and epigenetics mechanisms of multiple disease states. Single-cell studies and data analyses are being performed to generate a single cell transcriptome and epigenome atlas of human brain regions such as prefrontal cortex, striatum, and hippocampus. Commonly used methods in the laboratory include large scale functional perturbation studies using RNAi and CRISPR, Simultaneous single-cell RNA sequencing and single-cell Assay for Transposase- Accessible Chromatin sequencing (scMultiome-seq), patient-specific stem cell derived brain and lung organoids, drug design and pharmacology, and analyses of immune cells’ functions.