Single-Cell Physiology.

TitleSingle-Cell Physiology.
Publication TypeJournal Article
Year of Publication2015
AuthorsTaheri-Araghi S, Brown SD, Sauls JT, McIntosh DB, Jun S
JournalAnnu Rev Biophys
Volume44
Pagination123-42
Date Published2015 Jun 22
ISSN1936-1238
Abstract

Single-cell techniques have a long history of unveiling fundamental paradigms in biology. Recent improvements in the throughput, resolution, and availability of microfluidics, computational power, and genetically encoded fluorescence have led to a modern renaissance in microbial physiology. This resurgence in research activity has offered new perspectives on physiological processes such as growth, cell cycle, and cell size of model organisms such as Escherichia coli. We expect these single-cell techniques, coupled with the molecular revolution of biology's recent half-century, to continue illuminating unforeseen processes and patterns in microorganisms, the bedrock of biological science. In this article we review major open questions in single-cell physiology, provide a brief introduction to the techniques for scientists of diverse backgrounds, and highlight some pervasive issues and their solutions.

DOI10.1146/annurev-biophys-060414-034236
PubMed URLhttp://www.ncbi.nlm.nih.gov/pubmed/25747591?dopt=Abstract
Alternate JournalAnnu Rev Biophys
PubMed ID25747591
Track(s): 
Bioinformatics and Systems Biology